Biosensing methods and devices using graphene or graphene oxide (GO) have recently been developed with specific detection from body fluid samples, such as saliva, milk, urine, and serum. Graphene and GO have also been shown to have promising applications in other biomedical researches, such as photothermal treatment of cancer, drug and gene delivery, and cellular imaging. All these applications in practice will have to contact with the body fluid. As lysozyme (1,4-�-N-acetylmuramidase) is an abundant protein in the body fluid, one important question is to address how GO interacts with lysozyme. In this study, GO has demonstrated huge quenching effect on fluorescence of lysozyme, but it only slightly quenches other proteins, such as serum albumins, insulin, and amyloid � 40. The nature of this strong interaction between GO and lysozyme is found to be electrostatic interaction from studies of fluorescence quenching, dynamic light scattering, zeta potential, and atomic force microscopy. This interaction is so strong that we are able to selectively load lysozyme on GO.

Strong and selective adsorption of lysozyme on graphene oxide

247th National Meeting (2014)

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