Fluorescent dyes conjugated to polymers are used in order to visualize these materials in a biological environment. However, when conjugating a dye to a polymer a Poisson distribution of dyes per polymer particle is obtained. A generation 5 poly(amidoamine) (G5 PAMAM) dendrimer has the capability of having the distribution of hydrophobic ligands (rhodamine dyes) separated based on number by using a reverse phase semi-preparative high performance liquid chromatography (rp-HPLC) . Materials with either neutral or positive surfaces have been separated providing material with precise numbers of carboxytetramethylrhodamine (TAMRA) dyes and their individual characteristics have been characterized . The presentation will also include data from biological studies.

Characterization and application of dendrimer-rhodamine conjugates

247th National Meeting (2014)

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