Conductive polymers are versatile transducers utilized in diagnostic technologies. The chemical or electrochemical polymerization processes and the bio-conjugation of the synthesized polymers on a surface can be well controlled to endow them with desirable functions. We devised a conductive polymer based quantitative potentiometric biosensor that offers great assay capability. The sensor allows the ultrasensitive and rapid detection of analytes in complex physiological medium with a wide dynamic range. Polyaniline on electrode surface was enzyme labeled with an immuno-complex through thiol or glutaraldehyde based conjugation on the benzoid amine or quinoid imine moieties of its repeating units. The detection is mediated by the direct redox reaction between the polymer and charged product generated through the enzymatic reaction. Figure 1. <img src='https://acs-archive-assets2.s3.amazonaws.com/240nm/uploads/43290_figure1.jpg' alt='' /> The magnitude of the electrical response was found as a function of the polymer layer thickness, dopants and nanostructure. This polymeric bio-sensing approach is proven viable in the traditional open circuit potentiometric system and novel FET-based biochip systems.

Conductive polymer based ultrasensitive potentiometric immunosensors

247th National Meeting (2014)

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