Metal organic frameworks, or MOFs, are porous crystalline structures consisting of metal ion building blocks connected to organic linkers. MOFs that possess large pores and high surface areas can be created through manipulation of these building blocks. These types of MOFs are ideal for enzyme immobilization. The targeted enzyme in this project is Microperoxidase-11(MP-11). Aggregation of MP-11 hinders the enzyme's ability to oxidize organic molecules. Immobilization in a protective matrix will permit MP-11 to maintain its catalytic properties. For successful immobilization to take place, special attention must be paid to the parameters of the matrix chosen. STU-1, a mesoporous MOF that consists of Zn2+ metal centers and a bridged imidazole based linker has thermal stability, overall chemical robustness, and will be used in this study of the immobilization of MP-11. STU-1 has a pore size of 27.3 � x 15.4 �, which can tightly hold the MP-11 enzyme (~17.5 � x 33 �).This snug fit will protect the enzyme from the reaction medium, extending the life of this catalyst. In this work the immobilization of MP-11 will be verified and observed using Ultraviolet Visible Spectroscopy (UV-Vis).

Mesoporous metal organic frameworks for use in enzyme immobilization

247th National Meeting (2014)

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