Natural protein (silk fibroin) nanoshells were assembled on the surface of Saccharomyces cerevisiae yeast cells without compromising their viability. The nanoshells facilitated initial protection of the cells and allowed them to function in encapsulated state for some period of time, followed by complete biodegradation and consumption by cells. Gentle ionic treatment stabilized silk fibroin structure in the shell, and preserved the viability of the cells, as indicated by the fast response of the encapsulated cells, with an immediate activation of green fluorescent protein by the inducer molecules. Extremely high viability rates (up to 97%) and preserved activity of encapsulated cells are facilitated by cytocompatibility of the natural proteins and the formation of highly porous shells in contrast to traditional polyelectrolyte-based materials. Moreover, in a high contrast to traditional synthetic shells, the silk proteins are biodegradable and can be consumed by cells at a later stage of growth, thus releasing the cells from their temporary protective capsules.

Cell surface engineering with silk protein nanoshells

247th National Meeting (2014)

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