Bone morphogenetic protein 2 (BMP-2) is a member of the TGF-� growth factor family that exhibits multiple functionalities in the process of bone remodeling.  As such, surface modification of intrinsically bioactive ceramic scaffolds with BMP-2 could serve to further enhance the osteogenic effects of the scaffolds at a bone defect site.  In this study, calcium aluminate and hydroxyapatite scaffolds were modified with BMP-2 by direct surface adsorption or chemical immobilization utilizing an organic linker system.  The effect of surface modification was evaluated through the use of a live/dead cytotoxicity assay, which evaluated cell viability at days 1, 4 and 7. In addition, scaffolds were analyzed using scanning electron microscopy to evaluate pore configuration and other morphological features, as well as well as human osteoblast growth. It was shown that despite CaAlO having a more favorable pore configuration, BMP-2 immobilized on the surface of HA promoted cell viability to the highest degree.

Analysis of osteoblast morphology using scanning electron microscopy

247th National Meeting (2014)

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