A primary tool in DNA repair is the base excision repair (BER) process.  What initiates this process is not fully understood but a dynamic component has been suggested.  Deuterium labeled nucleosides have been synthesized, incorporated into a well-known DNA sequence (the Dickerson dodecamer), and analyzed via comparative solid-state NMR of damaged and normal DNA.  The NMR analysis is able to provide information about the local flexibility of these labeled sites.  A deoxyadenosine nucleoside with the base containing an etheno lesion and deuterium label has been synthesized, along with a base labeled control.  Solid state NMR data collected for these compounds can provide insight into whether the etheno lesion causes changes in the local dynamics of DNA.  These changes could indicate that promotion of the BER process is dependent on DNA repair proteins detecting flexibility differences of the DNA helix.

Dynamic analysis of DNA containing etheno adenine using deuterium solid-state nuclear magnetic resonance

247th National Meeting (2014)

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