The binding interactions of NAMI-A, [ImH][trans-RuCl4(DMSO)(Im)], with RNA and DNA were investigated using fluorescence spectroscopy under a variety of pH and reducing environments.  Both internal and external fluorophores were used to distinguish between binding locations, e.g., major vs. minor groove of DNA. Measurements of the Y base fluorescence of tRNAPhe indicate that NAMI-A disrupts the normal tertiary structure surrounding the Y base.  We will present the conditions that favor Ru-RNA binding, comparisons between Ru-RNA and Ru-DNA binding, and the role of cooperativity.

NAMI-A binding interactions with RNA and DNA

248th National Meeting (2014)

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