In the presentation, I will introduce a new gene delivery system, NanoSubstrate-Mediated Delivery (NSMD) platform, based on two functional components, including 1) DNA�SNPs: supramolecular nanoparticle (SNP) vectors for encapsulation of gene, and 2) Ad-SiNWS: adamantane (Ad)-grafted silicon nanowire substrates. The multivalent molecular recognition between the Ad motifs on Ad-SiNWS and the �-cyclodextrin (CD) motifs on DNA�SNPs leads to dynamic assembly and local enrichment of DNA�SNPs from the surrounding medium onto Ad-SiNWS. The topographic interactions between SINWS and cells result in a highly efficient delivery of exogenous genes. In addition, NSMD system enables continuous and multi-round delivery of the gene using the same substrates without depositing payloads and immobilizing cells in each round of delivery. Using the NSMD platform in vivo, cells recruited on subcutaneously transplanted Ad-SiNWS were also efficiently transfected with exogenous genes loaded into SNPs, validating the in vivo feasibility of this system.

Highly efficient nanosubstrate-mediated delivery of gene-encapsulated nanoparticles based on molecular recognition

248th National Meeting (2014)

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