In microalgal biofuels arena, nutritional culture conditions play major roles in optimal biomass productivity and material input/output balances. In this study, effects of various macro- and micronutrients on the growth rate and neutral lipid accumulation of four different microalgae species were investigated. For each nutrient, varying concentrations were compared to most commonly utilized media recipes. Biomass growth and photosynthetic activity were monitored via high-throughput screening (HTS) assays. Neutral lipid accumulation has been determined with specific fluorescent dyes and confocal laser microscopy imaging techniques. Tested nutrient conditions were validated in scaled up reactors where similar or better lipid productivities per unit volume were observed. Extensive optimization of macro- and micronutrient concentrations offered several benefits of decreased material input, waste generation, and overall costs.

Effects of macro- and micronutrients on neutral lipid accumulation in oleaginous microalgae

248th National Meeting (2014)

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