Monosaccharide and disaccharide determinations are important to various industries from ensuring quality product to researching biological pathways and disease states. Because carbohydrates are poor chromophores, chemical derivitization is needed for absorption. However, derivitization is costly, labor-intensive and may cause changes in molecular configuration. High Performance Anion-Exchange chromatography with Pulsed Amperometric Detection (HPAE-PAD) is a proven sensitive method to directly and selectively determine carbohydrates. In HPAE-PAD, carbohydrates are ionized in strong base and separated by anion-exchange chromatography. The carbohydrates are detected by PAD with a gold working electrode using a four-potential waveform selective and sensitive for carbohydrates. This sensitivity allows carbohydrate analysis down to pmole concentrations or when the samples are limited. This sensitivity is moderated in beverage samples which contain g/L concentrations by minimizing the flow path combined with moderate dilution. Here we combine the advantages of a reagent-free capillary format IC to determine monosaccharides and disaccharides in various applications, from low concentrations in synthetic urine samples to high concentrations in beverage samples. In a reagent-free IC system, the hydroxide eluent is electrolytically generated inline to deliver accurate and precise concentrations for isocratic or gradient separations by only adding deionized water. Eluent generation eliminates carbonate contamination and errors from manual preparation. A capillary scale system with �L/min flow rates can run 24/7, always on and always ready for samples. Eluent consumption and waste generation are reduced to 5.2 L/yr and eluent generator cartridges can last up to 18 months.

Using capillary HPAE-PAD to determine carbohydrates in various matrices

248th National Meeting (2014)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.