Background and Objective : Food allergy has been a serious problem that affects more and more people's life quality. Nowadays, although there are many in vitro methods to evaluate proteins' allergenicity, the reliability and viability of in vivo animal experiment has been suspended for a long time. To research the feature of different animals, we compared allergy reaction to peanut protein extract (PPE) between BALB/c and C3H/HeOuJ mouse.Methods : PPE was gavaged to BALB/c and C3H/HeOuJ mice with dose of 6mg/mouse, once a week and for 4 weeks. The control animals were gavaged with PBS. All animals were challenged at the end of sixth week with 30mg PPE. And then the PPE specific IgG1 and IgE, the histamine and mMcp-1 in different groups were compared. With addition, the ratio of Th-1 and Th-2 cell in every group was also investigated. At last, the manifestation and body temperature change of the treated animals were evaluated.Results:  After oral administration with PPE, levels of specific antibodies and Th- cell subsets in BALB/c mice were higher than that in the C3H/HeOuJ mice. On the contrast, allergic manifestation and decrease of body temperature in BALB/c mice were significantly lower than C3H/HeOuJ mice. This demonstrates that humoral response and cellular immunity to allergic food in BALB/c mice are more intense than that in C3H/HeOuJ mice. However, the allergy symptoms in C3H/HeOuJ mice are much more severe than BALB/c mice.Conclusions : The different reactions between two strains of mice after same sensitization protocol imply that different animals have special priority to evaluate foods' allergenicity. Maybe it is scientific to comprehensive confirm the allergenicity of a food with an evaluation system composing of a variety of animals. And this research can provide some reference for the establishment of animal model system.

Comparison of allergic reaction between BALB/cmouse and C3H/HeOuJ mouse after oral gavage with peanut protein

248th National Meeting (2014)

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