A survey of 100 wheat fields was conducted across Golestan province-Iran to investigate the ALS-inhibitor resistance in Sinapis arvensis L. Seeds were collected from fields and planted into the pots in greenhouse. The study was carried out in two steps including screening wild mustard biotypes with discriminating dose and determining resistance factor by dose-response bioassays in greenhouse conditions. After screening test for 40 suspected resistant biotypes collected during two years (2012-2013), 17 biotypes were recognized as resistant (R). In dose-response studies, five seedlings from R biotypes along by a susceptible biotype (S), from regions with no chemical control record, at the cotyledon stage were transplanted into the pots. The plants were sub-irrigated and grown to the four-leaf stage under supplemented natural light in the greenhouse. In four-leaf stage, a foliar application of tribenuron-methyl for S and R biotypes was made, equivalent to 0, 0.25, 0.5, 1, 2, 4, 8, 16 and 32 times of the regular field use rate or  0, 3.75, 7.5, 15, 30, 60, 120, 240 and 480 g a.i. ha-1. Four weeks after spraying, plants were harvested and the shoot dry weight measured. Results confirmed that the R biotypes were 2 to 9 fold resistant to tribenuron-methyl compared with the susceptible biotype.

Investigating resistance of suspected resistant biotypes of wild mustard (<i>Sinapis arvensis L.</i>) to tribenuron-methyl collected from wheat fields of Iran

248th National Meeting (2014)

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Investigating resistance of suspected resistant biotypes of wild mustard (Sinapis arvensis L.) to tribenuron-methyl collected from wheat fields of Iran