Pesticides play an important role in sustaining agricultural production by protecting crops from pest attacks and by keeping the pest population under control. Availability of safe and efficacious pesticides and their judicious use by the farming community is critical to a sustained increase in agricultural production and productivity. Keeping pace with the vision of the organization for a sustainable tomorrow, TERI under the patronage of Department of Biotechnology has developed an Eucalyptus plant extract based bio pesticide formulation �Bollcure� having proven efficacy for usage against a variety of pest complex. Leaf material of Eucalyptus camaldulensis was collected and shade dried and crushed into powder in a mixer grinder for bioassay studies and preparation of the extracts. Extraction of the leaf powder was carried out in soxhlet apparatus Fractions were obtained on silica gel column eluted with hexane and ethyl acetate gradient. The active fractions obtained by column were purified by RP-HPLC. Purified compounds were identified by mass spectrum and H1NMR and C13NMR. More than eighty per cent growth inhibition with slow growth and development of larvae was observed. These findings prompted us to do further fractionation and isolation of bioactive compounds and develop the extract and fraction based formulations, and carry out laboratory evaluation to see whether the activity is retained in the formulations or not. We formulated the extract based EC formulations registered as Bollcure. It has been found effective against Helicoverpa armigera, and other pest complex like Spodoptera, DBM, Cabbage looper, Silver white fly, Melon aphid, Western flower thrips, Cabbage aphid, Asian citrus psyllid, Root knot nematode, Thrips, Jassids, Mealy Bug.

TERI-DBT Bollcure: A new botanical biopesticide from Eucalyptus leaves

248th National Meeting (2014)

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