The resistance mechanism of highly (HR) and moderately (MR) carbendazim-resistant strains of Fusarium graminearum was investigated. Sequencing analysis of homologous �1 and �2-tubulin genes revealed two point mutations at codons 6 (A6N) and 200 (F200Y) in the �2-tubulin gene present in isolates from the MR phenotype. Isolates bearing the novel A6N substitution could not express their resistant phenotype at low temperatures. No point mutations were indentified in HR strains which had identical deduced amino acid sequences of the �1 and �2- tubulins with those of the wild-type strain. Real-time PCR assays from cultures of strains grown in presence or absence of carbendazim showed that the relative expression levels of homologous �- tubulins were not-correlated with carbendazim resistance, indicating that the resistance mechanism of HR strains is other than target site modification or overexpression of the �-tubulin gene(s).

Molecular studies on benzimidazole-resistance in <i>Fusarium graminearum</i>

248th National Meeting (2014)

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Molecular studies on benzimidazole-resistance in Fusarium graminearum