An aptamer- and FRET-based assay for one-step (i.e. no requirement of pretreatment) quantitation of target molecules of interest has been developed. This method has been successfully employed for the fast and sensitive detection of hepatitis B virus surface antigen (HBsAg). Key features of our method include its low intrinsic background noise, low costs, high resolution, and high sensitivity, enabling detection of as low as 1.25 mIU/mL, approximately 40-fold better than that of the most widely used Abbott Architech assay for HBsAg detection, without the tedious extraction and/or washing procedures. Moreover, this assay has better recovery and accuracy than that of conventional competitive binding assay or others for HBsAg quantitation.

One-step quantitation of hepatitis B surface antigen based both on aptamers and fluorescence resonance energy transfer

248th National Meeting (2014)

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