Plant growth regulators are synthetic organic compounds which are able to control plant physiological processes. They are classified into auxins, gibberelllins, cytokinins, inhibitors, and ethylene. They are used for preventing lodging in cereals and pre-harvest fruit drop, synchronizing maturity to facilitate mechanical harvest, hastening maturity to decrease turnover time, reducing labor requirements, etc. Forchlorfenuron is a cytokinin which improves fruit size, fruit set, cluster weight and the ability for low-temperature storage of grapes and kiwifruit. Inabenfide inhibits gibberellin biosynthesis which leads to a shortening of lower interlodes and upper leaf blades, so it is used to increase resistance to lodging in rice. This study was conducted to develop improved analytical methods for forchlorfenuron and inabenfide. We have selected representative agricultural commodities as brown rice, soybean, potato, pepper, and mandarin to validate this methods. First of all the extraction solvent considered as acetone to those two analytes. After suction filtration, rotary evaporation applied followed by liquid-liquid extraction to remove interferences. Finally we used a florsil cartridge for forchlofenuron and NH2 cartridge forinabenfide. The maximum absorbance was observed forforchlofenuron at 262 nm and for inabenfide at 271 nm in HPLC/UVD. Based on Codex Guideline 40: 1993, method validation was done against above five agricultural commodities. Matrix matched recovery measured at 1, 2, and 10 times LOQ(0.05, 0.1. 0.5 mg/kg). The recovery testing repeated by five times at each matrices and concentrations. We didn't find out any interference around analyte peaks in the chromatograph. The observed recoveries ranged 79 ~ 109 % for forchlorfenuron and 74 ~ 107 % for inabenfide. The repeatability was less than 10% at all conditions. Finally, we could confirm the results of HPLC/UVD with LC-MS/MS. Therefore, we could think that these analytical methods are the most appropriate to forchlorfenuron and inabenfide residues in agricultural commodities.

Establishment of analytical methods for forchlorfenuron and inabenfide, plant growth regulators, in agricultural commodities using HPLC/UVD

248th National Meeting (2014)

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