Many cellular processes are carried out by large protein complexes that can span several tens of nanometers. Whereas Forster resonance energy transfer (FRET) has a detection range of <10 nm, here we report the theoretical development and experimental demonstration of a new fluorescence imaging technology with a detection range of up to several tens of nanometers: Singlet oxygen Triplet Energy Transfer (STET). We demonstrate that STET confirms the topology of a large protein complex in intact cells, which spans from the ER to the outer mitochondrial membrane and the matrix. This new method is thus suited for mapping protein proximity in large protein complexes.

Singlet oxygen triplet energy transfer based imaging technology for mapping protein-protein proximity in intact cells

248th National Meeting (2014)

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