Antrodia camphorata is a species known to be available only in Taiwan. Many researchers have investigated its polysaccharides. However, no distinctive polysaccharide has been identified yet. In this study, we isolated a mycelial strain NPU-50 from a natural fruiting body of A. camphorata. Crude polysaccharides produced byNPU-50 in solid-state fermented brown rice were extracted and then applied to a gel filtration column equipped with a HPLC-RI detector for purification. The results indicated that the fermented product consisted of 3 major polysaccharides with various molecular weights of 290 kDa (designated as ACP-1), 8.1 kDa (ACP-2) and 1.7 kDa (ACP-3). During the cultivation, ACP-1 increased, while ACP-2 and ACP-3 remained unchanged or diminished. The results from GC and GC-MS indicated that ACP-1 was composed of fucose, arabinose, mannose, galactose and glucose at a ratio of 5:5:22:43:104. The glycosidic bonding between monosaccharides in the ACP-1 were mainly 1,6-galactose linkages. The 1H-NMR spectrum indicated that ACP-1 consisted of a-D-mannose and �-D-galactose. ACP-1 stimulated proliferation of peripheral blood mononuclear cell by 6.1 fold comparing with non-treated group. In addition, we also utilized ACP-1 to induce a polyclonal antibody (designated as anti-ACP-1) using a rabbit. During immunodiffusion analysis, an anti-ACP-1 precipitation band was observed only in the presence of ACP-1, but not in the presence of ACP-2, ACP-3 or lipopolysaccharides. Meanwhile, the anti-ACP-1 only performed immune reaction with NPU-50, but not with other fungi, such as Saccharomyces cerevisiae or Rhizomucor miehei, using Western blotting. Taken together, our results provide a molecular basis to describe at least part of the characteristics of a unique polysaccharide ACP-1, and suggest the possibility of using the polyclonal antibody anti-ACP-1 as an instrument for assessing the genuineness and quality of A. camphorata NPU-50 fermented products.

Characteristics and polyclonal antibody of a novel polysaccharide ACP-1 produced from mycelial strain NPU-50 of <i>Antrodia camphorata</i>

248th National Meeting (2014)

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Characteristics and polyclonal antibody of a novel polysaccharide ACP-1 produced from mycelial strain NPU-50 of Antrodia camphorata