The ADAR enzymes convert adenosines to inosines in RNA in an example of base modification RNA editing.  Since inosine is decoded as guanosine during translation, this modification can lead to changes in the meaning of codons (recoding).  At least 50 recoding events are known in human mRNAs and perturbations in A to I editing have been observed in several human diseases.  Recent efforts to define the basis for selectivity in the ADAR reaction will be described including experiments that use RNA containing the nucleoside analog 7-ethynyl-8-aza-7-deazaadenosine (7-EAA).  7-EAA in RNA is both a substrate for a mutant of human ADAR2 as well as a substrate for copper-catalyzed azide-alkyne cycloaddition reactions.

Chemical approaches to the study of A to I RNA editing

248th National Meeting (2014)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.