Detection of intercellular or extracellular pH changes have been studied in biological and biomedical applications as well as chemical and environmental applications. Because condition of tumor and inflammation is generally acidic, knowledge of decreasing pH provides essential information about diseases. Also, sensing of intercellular glutathione (GSH) that is the most abundant cellular thiol and plays very important role in organisms is useful to understand processes of many diseases.We developed cyanine conjugated silica nanoparticle NIR fluorescent probe for detection of pH changes and synthesis remarkably sensitive GSH cyanine-based NIR fluorescent chemosensor. Fluorescent nanomaterials have some advantages including enhanced permeability and retention effect compared to free dye. We synthesized silicon nanomaterial fluorescent probe with a size of 5-10nm and 15-20nm that can be used biomedical imaging of pH changes. For near infrared (NIR) range fluorescence, cyanine structure has been used. NIR fluorescence can penetrate deeply into tissues and moreover, less damage biological sample. In these reasons NIR fluorescent probes are proper to understand biological mechanisms and can be used in many applications. Importantly, these fluorescent probes can be utilized to monitor the GSH in mouse tissue cells as well as in cell cultures.(1) Guo, Z.; Park, S.; Yoon, J.; Shin, J. Chem. Soc. Rev. 2014, 43, 16.(2) Chen, X.; Zhou, Y.; Peng, X.; Yoon, J. Chem. Soc. Rev. 2010, 39, 2120;

Cyanine conjugated silica nanoparticles as NIR fluorescent probes for bioimaging applications

248th National Meeting (2014)

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