Pyflubumide is a novel and potent acaricide invented and under development by Nihon Nohyaku Co., Ltd. The molecule can be classified as carboxanilides and its structural similarity to those of fungicidal succinate dehydrogenase inhibitors suggested the target site of this molecule might also be mitochondrial complex II. Accordingly mitochondrial complex II inhibitory potential of pyflubumide and its metabolite was examined. A pyflubumide metabolite exhibited high inhibitory activity on the mitochondrial complex II, but did not show any additive inhibitory activity with the active form of cyenopyrafen in double-inhibitor titration assays. These results suggested that high acaricidal activities of pyflubumide can be attributed to the inhibition of the electron transport in mitochondrial complex II by its metabolite, and its binding manner to the site was different from that of active form of cyenopyrafen, beta-ketonitrile derivative.

Mode of action of novel acaricide pyflubumide: Effects on the respiratory electron transport chain

248th National Meeting (2014)

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