Several genes were identified as biomarkers of the fipronil resistance in the backcrossed progenies of the fipronil resistant silkworms. We checked the gene expression by transcriptome and performed LDA with the 91 loci that showed more than 5 fold changes. Four out of the 91 loci (figure 1, table 1) were potentially unsuitable to judge the fipronil resistance because of higher rate of false in multiple combinations of the loci than the other loci. More than 55 loci were necessary to achieve accurate judgments (rate of failure = 4.0000E-05) of the fipronil resistance. The rate did not dramatically improve when 3 to 34 loci were used in the analysis (rate of failure = 0.0001 ~ 0.0006). This shows that we can screen the fipronil resistance by the expression profiles of the 3 genes as biomarkers at a success rate 0.9999.Genomic sequencing of the silkworms revealed no mutations that could induce the resistance on the candidates of the targets such as GABA-gated chloride channels (Hosie et al. 1995) and the glutamate-activated chloride channels (Ikeda et al. 2003). The transcriptome of this study can be a clue to understand the fipronil resistance of the silkworms.

Biomarkers of the fipronil resistance

248th National Meeting (2014)

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