Affinity chromatography has been used to evaluate apparent binding constants for divalent metal ions to phospholipid bilayers. The analytical format utilizes lipids supported on newly developed solid porous particles within fused silica capillaries with ICP-MS detection. The relatively high throughput of the technique facilitates the comparative measure of a number of factors that affect observed binding behavior, including lipid type, ionic composition of the mobile phase, and the presence of mobile phase additives known to affect lipid bilayer properties (e.g., alcohol). Issues unique to the analysis of modest-affinity interactions with ionic analytes by frontal analysis methodology have been revealed that place some constraints on applicable experimental conditions.

Affinity measurements between divalent metal ions and phospholipids by analytical affinity chromatography

248th National Meeting (2014)

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