The use of fenamidone, an imidazolinone fungicide, has increased in Costa Rica since 2006 .The pesticide is commonly used for the control of fungal diseases in potatoes, melons and cucurbits. In order to determine if the compound has also been applied in pineapple production, a crop in which the use is not registered, a method to analyze its residues was urgently needed.This work reports the validation of a modified QuEChERS method for the analysis of fenamidone in pineapple.The method was implemented using ethyl acetate (EtOAc) as a solvent instead of acetonitrile (MeCN) for the extraction [1]. For the validation and in comply with quality standards of ISO/IEC 17025, the fruit was analyzed as a whole (without the crown) and its parts such as peel, pulp and core (fleshy axis) were also analyzed separately. For the extraction 10 g of the homogenized sample was mixed with 1.7 g NaHCO3and 20 ml of EtOAc, following manual agitation, 10 g anhydrous Na2SO4 and 1 g NaCl were added. The sample was centrifuged and the supernatant was cleaned up by dispersed solid phase extraction using MgSO4and PSA as absorbent. An aliquot of 10 ml of the clean extract was centrifuged and 1 �l of this extract was injected into the GC-MS/SIM.The validation of the method was performed using three concentration levels (0.03, 0.3 and 3 mg/kg) for all plant compartments. The recoveries were between 72 and 102 % with a relative standard deviation of 1-10%.  [1] Aysal, P., Ambrus, �.,Lehotay, S and Cannavan A. Validation of an efficient method for the determination of pesticide residues in fruits and vegetables using ethyl acetate for extraction. J. Environ. Sci. Heal. B. 2007. 42, 481-490.

Validation of a QuEChERS and GC-MS/SIM method for fenamidone residues analysis in pineapple

248th National Meeting (2014)

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