Studies on Coenzyme Q10 (CoQ10) have shown its potency against heart related diseases in humans but due to high molecular weight and hydrophobicity, it has low bioavailability. To improvise its efficacy, CoQ10 loaded cocoa butter solid lipid nanoparticles (CBSLN) with loading range from 0.5% to 2% (w/w) were developed using high-speed and pressure homogenization of pure cocoa butter (CB) with polysorbate and sorbate monostearate 60 as emulsifiers at 55?C and stored at 4?C. Particle size analysis, zeta potential and stability studies were performed by dynamic light scattering method. The novel CoQ10-CBSLN dispersions had non-ionic nanoparticles with average size 200 nm with 0.3 poly-dispersity index and possessed good stability at low and room temperatures. Due to high solubility of CoQ10 (110�4.7% w/w) in CB, 85-90% entrapment efficiency was achieved in their SLN form. DSC analysis of lyophilized CoQ10-CBSLN and their components showed that CB in SLN form exhibited polymorphism enabling co-crystallization with CoQ10. Sterilization process had major effects on the size and entrapment efficiency, confirming that CB recrystallization is influenced by cooling rates. On analyzing the SAXS intensity profiles of CBSLN and CoQ10-CBSLN, it was concluded that there is a slight decrease in thickness of the interface due to increase in loading. Bioaccessibility of CoQ10 in mixture of CoQ10 and CB, emulsified CoQ10 (with same surfactants used in SLN) and CoQ10-CBSLN were assessed and compared using in-vitro lipolysis and HPLC analysis of the aqueous and insoluble phases. Bioaccessibility due CoQ10-CBSLN of different loading was eight times greater than that of emulsified CoQ10 and six times in case of mixture of CB and CoQ10. These results suggest that micelles formed from fatty acids released by increased lipolysis rate of CBSLN can act as vehicles to carry CoQ10 to make it more accessible for gastro-intestinal absorption. This nano-delivery system can be extended to encapsulate other hydrophobic bioactives.

Development and characterization of cocoa butter solid lipid nanoparticles to improve bioaccessibility of coenzyme Q10

248th National Meeting (2014)

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