Nanocomplexes assembled from ionic crosslinking of cationic chitosan (CS) and anionic caseinophosphopeptides (CPPs) exhibit the advantage of improving the bioavailability of hydrophilic nutraceuticals, such as black tea flavonoid theaflavin-3,3'-digallate (TF-3). Systematic studies of interactions between TF-3, CPPs, and CS at physiological environment by fluorescence quenching, turbidimetric titration, quartz crystal microbalance with dissipation monitoring (QCM-D) and grazing angle FT-IR revealed that hydrogen bonding is the predominant force between CPPs and TF-3 both in aqueous phase and solid-liquid interface, and electrostatic interaction dominates the interaction between CS and CPPs. The particle size of TF-3 loaded Chitosan/CPPs nanocomplexes increased from 200 to 300 nm gradually as corresponding to the increased amount of loaded TF-3. Cellular transport study using Caco-2 cell line showed that this novel delivery system effectively enhanced the permeation rate of encapsulated TF-3, and thus improved the in vitro bioavailability of TF-3. These results provide a better understanding of how this food-grade delivery system exists in physiological condition and give a prospective of further use in improving the bioavailability of other hydrophilic nutraceuticals.

Design of novel chitosan/caseinophosphopeptides nanocomplexes-based delivery system to enhance bioavailability of theaflavin-3,3'-digallate

248th National Meeting (2014)

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