A novel biocompatible magnetic cellulose nanocrystals (MCNCs) composite was in situ prepared via a co-precipitation-electrostatic-self-assembly technique and successfully used for immobilization of papain with great application potential. The anionic cellulose nanocrystals (CNCs) were effectively composited with cationic chitosan-coated Fe3O4 by the electrostatic interactions between CNCs and chitosan, as well as between chitosan and Fe3O4. Papain immobilized on the prepared MCNCs exhibited remarkably higher thermostability, storage stability, pH-stability and organic solvent tolerance than free enzyme. The optimum range of pH (5-10) and temperature (30-80oC) for the immobilized papain greatly surpassed that of free enzyme. Besides, the kinetic study showed that the catalytic efficiency of papain was significantly enhanced by immobilization on the MCNCs. Furthermore, papain immobilized on the MCNCs was very easily recycled from the reaction system using an efficient and convenient separation method of magnetic interaction, and manifested very competitive and promising for the enzymtic process.

Preparation of novel magnetic cellulose nanocrystals via in-situ co-precipitation-electrostatic self-assembly technique and its efficient use for enzyme immobilization

248th National Meeting (2014)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.