Analysis of single cells provides powerful insight into biological processes that are often missed when a population of cells is studied as an ensemble. We are developing microfluidic-based approaches coupled with optical microscopy to track individual bacteria and to improve the temporal and spatial resolution of single-cell measurements. To streamline our experiments, we designed and tested a microfluidic �baby machine,� which automates the culture, synchronization, and analysis steps. This microfluidic device is used to produce synchronized populations of cells and monitor adhesion of individual bacteria to surfaces. To better understand how bacteria attach to surfaces, rates of attachment of mutant strains that lack pili and motility are compared to the rate of attachment of wild-type cells. To synchronize non-adherent cells and study cellular growth, we integrated nanochannel arrays into the microfluidic devices that physically trap bacteria. With these nanochannel arrays, we are able to study a number of bacteria strains and observe their aging and growth over multiple generations. Consequently, we are able to determine rates of cell division and accumulation of cellular damage as the bacteria age.

Studying adhesion and aging of individual bacteria on microfluidic devices

248th National Meeting (2014)

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