The urgent need for new antibacterial antibiotics has led to synthesis and screening approaches for novel bioactive compounds. Bacterial ribosome is a well established drug target for the development of antibacterial compounds. We have developed high-throughput capable assays for ribosome targeted drug discovery. One such assay examines the compounds ability to bind to a model ribosomal RNA A-site. We have also coupled this assay to other functional orthogonal assays. Such analysis can provide valuable understanding of the relationships between two complementary drug screening methods and could be used as standard analysis to rapidly correlate the affinity of a compound for its target and the effect the compound has on a cell.1.     Watkins, D.; Norris, F.A.; Kumar, S.; and Arya. D.P.  �A Fluorescence Based Screen for Ribosome         Binding Antibiotics�.  Analytical Biochemistry, 2013 ;434(2):300-7.2.     King, A.; Watkins, D.; Kumar, S.; Ranjan, N.; Gong, C.; Whitlock, J. and Arya. D.P. �Characterization of     Ribosomal Binding and Antibacterial Activities Using Two Orthogonal High Throughput Capable Screens�.  Antimicrobial Agents and Chemotherapy, 2013  Oct;57(10):4717-26.

Assay for ribosome binding drugs

248th National Meeting (2014)

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