Electrochemical DNA transducers offer a simple and inexpensive platform for the detection of microorganisms. A viable approach to build these biosensors is to use DNA probes that are specific to the target microorganism. Our research goal is the development of an electrochemical biosensor for the detection of Salmonella.Our research design is the modification of electrode surfaces using different molar ratios (25:75, 50:50 and 75:25) of a styrene:n-acryloxysuccinimide (Sty:NHS) copolymer to anchor Salmonella DNA and detect the hybridization of the complementary strand. The hybridization process is detected using 2-ferrocenylimidazo[4,5-b]phenazines (Fc-Imidazole) as a redox label. The electrochemical analysis was done using a three electrode system with a glassy carbon as the working electrode, Ag/AgCl (3M NaCl) as the reference and nichrome as the auxiliary. The GC electrode was modified with the corresponding copolymer and the Salmonella's probe. The modified electrode was exposed to the target DNA solution. An increase in the current density of Fc-Imidazole was measured (1.498 �A/cm2 to 2.754 �A/cm2) as the molar ratio of NHS in the copolymer was increased from 25% to 75% respectively. Fc-Imidazole proved to be an adequate redox label to detect the hybridization of complementary oligo sequences. Our current work focuses on the method optimization.

Electrochemical detection of DNA hybridization using 2-ferrocenylimidazo[4,5-b]phenazines as a redox label

248th National Meeting (2014)

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