Acute myocardial infarction (AMI) is a well-recognized angiocardiopathy that can be spotted by a continuous rise and fall of 'chemical biomarkers' in a clinical context. Biomarkers are then 'valuable indicators' of an impaired cardiac function, together with ischemic symptoms. Assay of Cardiac troponin T (cTnT) is now an indispensable element in the medical practice followed to diagnose and control AMI.Herein, we report a new sensing strategy based on pulsed streaming potential for direct determination of CTnT. The current biosensor consists of a patterned microchannel built with commodity thermoplastic cyclo olefin copolymer (COC).Specific binding of �avidin to biotin� on an �EDC-NHS� modified microchannel was used to tether biotinylated �anti-troponin T monoclonal Ab� to the target �CTnT�. Analytical response to this system was measured by changes in magnitude of the streaming potential and was correlated to the amount of analyte bound to the sensor surface. The selectivity of the created platform was tested successfully in serum samples.Different from the well-established biochemical assays adopted for cTnT, the current biosensor does not require labeling (fluorescent, electrochemical or radioactive). In addition, a stable quick response is attained under pulsed flow not steady state conditions, which permits a real time analysis. The linear region (1.9 - 6.5 �g/mL) matches with the clinically relevant concentrations of cTnT in human serum which has a cutoff value of 4 ng/mL.    Procedure for immobilizing cTnT on the surface of microfluidic channel.

Application of pulsed streaming potentials in plastic microfluidic channels for quantitative point-of-care determination of cardiac biomarkers

248th National Meeting (2014)

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