Genetically simplified nitrate reductases (SNaRs) were coupled with electron transfer mediators methyl viologen (MV) and bromophenol blue (BPB) in reducing nitrate. The SNaR1 consists of the fragments of Molybdenum-molybdopterin (Mo-MPT) binding site and nitrate reduction active site. The SNaR2 contains the Mo-MPT binding site and the nitrate reduction active site as well as the Heme-Fe fragment. A sol-gel 'Laponite' was used to retain the activity of SNaRs on different types of commercialized and self-assembled electrodes. Cyclic voltammetry and chronoamperometry were used to detect nitrate. Good selectivity and sensitivity were obtained with both SNaRs.

Simplified nitrate reductase based nitrate biosensor

248th National Meeting (2014)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.