Natural Rubber latex (NRL) is a colloidal complex with particles sizes varying from 5 to 5000 nm. Casting membranes produced using NRL can accelerate cells proliferation and induce revascularization1, similar when in multilayer films configuration.2 But to achieve organized multilayer films3 of NRL the particles sizes distribution should be reduced and controlled. A Nanolatex solution, with particles below 200 nm, was obtained performing a series of centrifugations and filtration on NRL. Multilayer films of nanolatex were then mounted with polyethylenimine (PEI) and polyallylamine hydrochloride (PAH) up to 10 layer pairs. The increase of the films thickness per deposited layer was detected by ellypsometry (PlasMos GmbH) using 632.8 nm laser bean focusing on the sample at a fixed angle of 45 degrees. PEI/Nano was thicker than PAH/Nano, which also reflected on the contact angles and roughness of both surfaces. Films of PEI/Nano presented angles of 82� and roughness of 3.3 nm, while PAH/Nano showed angles of 69� and roughness of 0.7 nm. Apparently, those surfaces different characteristics had some impact on the 3T3 cells reactions. Films of (PEI/Nano)5 and (PAH/Nano)5 on round glass slides were directly seeded with 103 3T3 cells and kept in culture conditions for 1, 3 and 7 days [DEMEM medium with 10% BCS and antibiotics at 37�C in 5% CO2]. On each culture day the cells were count by MTT assay, and photos were taken for morphological analysis. Casting of nanolatex was also analyzed and polystyrene culture well was used as control. On nanolatex casting the amount of cells were higher than control up to 3 days, but on (PAH/Nano)5 it was extended to 7 days. The multilayer configuration might have preserved the bioactivity for longer time. However, (PEI/Nano)5showed for 1 day a double amount of cells than control, but it got decreased on the following days. We assume those results were influenced not only by chemicals difference (PEI and PAH) but also by the surface characteristics.1. Mru�, F.; Netto, J. C.; Ceneviva, R.; Lachat, J. J.; Thomazini, J. A.; Tambelini, H., Evaluation of biocompatibility of a new biomembrane. Materials Research - Ibero-american Journal of Materials. 2004, 7(2), 277-283.2. Davi, C. P.; Galdino, L. F. M. D.; Borelli, P.; Oliveira, O. N., Jr.; Ferreira, M., Natural rubber latex LbL films: Characterization and growth of fibroblasts. Journal of Applied Polymer Science 2012, 125(3), 2137-2147.3. Decher, G., Fuzzy nanoassemblies: Toward layered polymeric multicomposites. Science 1997, 277(5330), 1232-1237.

In vitro characterization of cell viability on nanolatex multilayers films

248th National Meeting (2014)

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