Pyridinium amphiphiles � lipids, gemini surfactants, lipophilic polycations can act as efficient synthetic transfection systems with reduced toxicity in vitro and in vivo. Recently we introduced a second-generation pyridinium gemini surfactants through comprehensive tuning of the self-assembling process of these amphiphiles at the level of counterion, hydrophobic chain, added co-lipids, their nature and molar ratio. The current study presents the effects of blending these new pyridinium cationic gemini surfactants into cationic pyridinium lipid bilayers and its impact towards plasmid DNA compaction and delivery process in terms of transfection efficiency, cytotoxicity and internalization mechanism of resulted nucleic acid complexes.

Incorporation of gemini surfactants into pyridinium lipoplexes enhances transfection through endosomal membrane destabilization

248th National Meeting (2014)

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