Liposomes (lipid vesicles) have been widely used as nanoparticle systems for drug delivery, diagnostics, imaging and for basic research as model membranes. Incorporation of non-exchangeable lipid-fluorescent dyes into liposomes have allowed researchers to trace the distribution in biological systems and binding/uptake of liposomes into cells. A large number of lipid conjugated fluorescent dyes have been used for the generation of fluorescent liposomes, however fluorescent liposomes used by most researchers are not optimized for the amount of dyes to minimize self-quenching and for optimal fluorescent intensity. In this study the fluorescence behaviors for a number of DHPE-conjugated fluorescent dyes in different types of liposomes are investigated. The fluorescent intensity are monitored as a function of dye/lipid ratio for liposomes composed of fluid membranes as well as solid membranes. The results indicate a distinct difference in the fluorescence behavior for each dye in liposomes with different fluidity. The results is very valuable and will serve as a guideline for researchers to design their fluorescent liposome systems.

Studies of fluorescence behavior of lipid-fluorescent dyes in liposomes

248th National Meeting (2014)

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