There are a number of approaches to interface biomolecules with gold nanoparticles (AuNPs). Existing strategies include electrostatic absorption, chemisorption (taking advantage of the strong thiol-Au interaction), affinity-based non-covalent interactions (His-6/Ni-NTA, streptavidin/biotin interactions), and AuNP surface passivation followed by subsequent functionalization. These strategies, however, suffer from significant background absorption of the AuNPs to the biomolecules of interest. Established conditions also often require reagents that are unstable in aqueous solutions, harsh reaction conditions, and/or long reaction times. Our group has developed a rapid, chemoselective bioconjugation through the oxidative coupling of anilines and aminophenols. This reaction proceeds under mild conditions with fast reaction times. Progress towards adapting this oxidative coupling strategy to make well-defined and stable AuNP-bioconjugates will be discussed.

Integrating gold nanoparticles with biomolecules through the oxidative coupling of anilines and aminophenols

248th National Meeting (2014)

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