The goal was the optimization and validation of a method for determination of paraquat and diquat in cowpeas.  The optimized extraction was achieved using methanol containing hydrochloric acid solution 5 mol L-1 (6:4). The isotopically labeled analogues of the target analytes were used as internal standards. The mixture of slurry and extraction solvent was shaken for 2 min, 15 min in a water bath at 80 �C. After cooling down the mixture was centrifuged. The determination was performed by UPLC-MS/MS. Analytical solutions have been prepared/stored in polypropylene vessels. The validation was performed by analyzing blank cowpea spiked at 10, 20 and 50 �g kg-1, n=7 at each level. The linearity (r2), accuracy (recovery %), instrument and method LOD and LOQ, precision (RSD %) and matrix effect (%) were assessed. This method can be used as an efficient and fast method for the routine determination of paraquat and diquat.

Optimization and validation of single residue method for determination of diquat and paraquat by UPLC-MS/MS in cowpea

248th National Meeting (2014)

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