Acute toxicity, bioaccumulation, and elimination of the herbicide clomazone in earthworms (Eisenia fetida) were investigated. The LC50 of clomazone on earthworms for 48 h of exposure was 5.6 �g�cm-2 in the contact filter paper test, and were 174.9 and 123.4 mg�kg-1, respectively for 7 and 14 days of exposure by the artificial soil test. Clomazone was rapidly absorbed by the earthworms and the highest concentration were reached after 3 days of exposure at the concentrations of 9.0, 35.3 and 142.3 mg kg-1, respectively, when they were cultivated in soils containing 10, 40 and 160 mg/kg clomazone. Clomazone uptake correlated with exposure concentration. Clomazone was also rapidly eliminated and/or transformed to other metabolites, as evidenced by its rapid decrease in earthworms after reaching peak concentrations. After 14th day of exposure, clomazone in earthworm was rapidly declined to 2.93, 4.62, and 18.70 mg kg�1 respectively.About 74-80% of the bioaccumulated clomazone were eliminated within 1 day after exposed to clomazone-free soil.

Bioaccumulation and elimination of herbicide clomazone in earthworms (Eisenia fetida)

248th National Meeting (2014)

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