A robust and sensitive method was developed for the analysis of coumoxystrobin in apple and soil, based on solid-phase extraction (SPE) coupled to high performance liquid chromatography with diode array detector (HPLC-DAD). Residue of coumoxystrobin was extracted from apple and soil with acetonitrile and methanol-water, respectively, followed by SPE to remove coextractives, before analysis by HPLC-DAD. SPE procedures were performed on Florisil cartridges (500 mg, 3mL). The analytes from apple and soil matrix were eluted with petroleum ether- acetone (85:15 v/v, 4 mL). Limits of quantification of the method were 0.01 mg kg-1, and the mean recoveries ranged from 85 to 104% with the coefficient variations (CV%) of the method ranged from 1.43% to 4.95% at the three spike levels (0.01, 0.1 and 0.5 mg kg-1). The proposed method was successfully applied to the analysis of coumoxystrobin residue in apple and soil samples from an experimental field. The half-life of coumoxystrobin in soil from experimental field was 5.7 days (in Shandong). The final residue of the fungicide in apple and soil was lower than 0.01 mg kg-1 at harvest time. Direct confirmation of coumoxystrobin residues in samples was realized by liquid chromatography-mass spectrometry (LC-MS).

Determination of coumoxystrobin in apple and soil by SPE clean-up and high performance liquid chromatography with DAD detection

248th National Meeting (2014)

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