New IL-containing systems were developed for synthesis of long chain nucleoside ester catalyzed by Pseudomonas fluorescens whole-cells. Various ILs exerted significant but different effects on the bio-reaction. And their effects were closely related with both the anion and cations of the ILs. Of all the ILs-containing systems tested, 10% [BMI][PF6] -THF was most effective for the reaction, in which the initial rate, product yield and 5'-regioselectivity reached 2.34 mmol/L�h, 81.1% and >99%, respectively. Furthermore, SEM analysis revealed that ILs can alter the cell surface morphology, improve the permeability of cell envelopes and thus facilitate the mass transfer of substrates to the active cites of cell-bound enzymes. Our research demonstrated the potential of ILs as promising reaction medium for achieving highly efficient and regioselective whole-cell catalysis.

Catalytic behavior and cell morphology of whole-cell biocatalysts for highly regioselective synthesis of arabinocytosine laurate in ionic liquids

248th National Meeting (2014)

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