A UHPLC-QToF-MS method has been developed for the comprehensive screening and quantitation of 150 veterinary drugs and pharmaceuticals, in a variety of chicken and fish tissues, covering a wide range of chemicals listed by the EU Regulation 37/2010, as well as pharmaceutical environmental contaminants. The method was validated according to the Commission Decision 2002/657/EEC for quantitative screening methods. A generic sample preparation procedure was developed, including a solvent extraction step and a clean-up step, where a dSPE and a simple clean-up procedure with hexane were compared. UHPLC- QToFMS/MS was used for the final analysis, in broad-band Collision Induced Dissociation (bbCID) mode, providing information of the MS and MS/MS spectra, simultaneously. The analysis was carried out in less than 20 minutes, in both positive and negative ionization mode (two separate runs) and the resolving power was always over 30000. Bruker Target Analysis 1.3 and Data Analysis 4.1 software modules were used for data evaluation, taking into consideration the retention time, precursor ions and adducts, as well as bbCID fragment ions. According to Commission Decision 2002/657/EEC, two identification points are earned for each HR-precursor ion and transition product, and in combination with the retention time, sufficient identification is achieved. Matrix effects can be overcome, since mass accuracy of 2 ppm and isotopic fit of <50 mSigma was established. An important advantage of this method is that retrospective investigation of the data is available to look for the presence of additional target residues and their metabolites, which were not considered at the time of the analysis. The utilization of liquid chromatography hyphenated to the high resolution mass spectrometry proved to be a powerful tool for routine analysis of veterinary drugs and pharmaceuticals in food of animal origin, contributing to food safety under the EU food chain legal framework.This research has been co-financed by the European Union (European Social Fund � ESF) and Greek national funds through the Operational Program 'Education and Lifelong Learning' of the National Strategic Reference Framework (NSRF) - Research Funding Program: Heracleitus II. Investing in knowledge society through the European Social Fund.

Quantitative multi-residue method for 150 veterinary drugs and pharmaceuticals in meat using liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-QToF-MS)

248th National Meeting (2014)

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