Market place demands continue to require an increase in the number and size of field trial studies. Thus the speed with which the samples can be prepared for analysis is under constant scrutiny and must be evaluated.  Additionally, the ability to ship large quantities of samples around the globe is increasingly difficult and costly.  The use of LC in tandem with MS/MS continues to be a powerful tool in the quantitative analysis of small molecules as well as proteins. The much higher selectivity gained with this tool vis-�-vis LC/UV approaches has allowed method development, validation, and analysis times to be reduced. While the bottleneck of extracted sample analysis has been drastically reduced with these techniques, most residue studies still start with multi-gram samples, requiring large solvent volume extractions. These extractions are time-consuming and costly to prepare.  In our lab, we have been transitioning from 20 gram sample sizes, prepared using traditional extraction methods, to 100 mg sample sizes prepared using geno-grinders and 96 well plate formats. The  most difficult thing to achieve is the homogeneity of the sample (and thus sub-sample) selected and extracted.  In this presentation, data from field soil dissipation studies and multiple MOR studies are shown that compare the original high-mass/high-volume method to methods using 100 mg samples and geno-grinders with very low volumes of organic solvents for extraction.  The savings in terms of solvent used, disposal costs, time, and overall efficiency are also discussed.

Development and implementation of high throughput 96-well plate techniques for use in residue and soil dissipation studies

248th National Meeting (2014)

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