Validated methods for protein quantification by enzyme-linked immunosorbent assay (ELISA) are necessary for accurate reporting and analyses; however, developing methods for extracting and quantifying proteins from soil or other from environmental matrices can be difficult. Oftentimes the buffer required to extract immunoreactive protein from soil and the potential interference introduced by the soil matrix affect ELISA analysis.   Central to ELISA validation are experiments designed to characterize interference due to the soil matrix or extraction buffer, as well as to optimize recovery of the protein from the soil matrix.  Additional validation to determine accurate interpolation of the protein concentration at multiple dilutions and the use of well-characterized quality controls and acceptance criteria help ensure the method is robust and reliable.  A systematic approach for conducting comprehensive ELISA validation experiments necessary to ensure accuracy and reproducibility of the method will be presented.

Systematic approach for validating enzyme-linked immunosorbent assay (ELISA) methods for protein quantification from soil

248th National Meeting (2014)

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