Antibody-based sandwich ELISA has been widely used to detect novel transgenic proteins expressed in genetically modified plants because of its high specificity, high throughput and low cost. However, the selection of a complementary antibody pair for ELISA that is specific and suitable for detecting the protein in the presence of plant matrix can be challenging. With the AlphaLISA technology, a label-free, complementary antibody pair screening method has been developed which can directly screen mouse hybridoma culture supernatants at the earliest stage. The results demonstrated that the developed method is a robust, time- and cost-effective approach for selecting mouse monoclonal antibody pairs that are suitable for sandwich ELISA detection of transgenic plant proteins.

Development of an AlphaLISA-based complimentary antibody pair screening method for transgenic protein detection in maize

248th National Meeting (2014)

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