The synthetic method used in the preparation of commercially available stationary phases for chromatography is generally a trade secret. As such, the extent to which ATRP is used in the preparation of such stationary phases is not generally known. Even so, we believe that our lab leads the world in the use of ATRP as a synthetic tool for the preparation of engineered stationary phases on nonporous styrenic substrates. Currently we utilize ATRP in the synthesis of four different commercially available stationary phases and we are in the process of developing a fifth stationary phase using this technology. ATRP provides considerable advantages over conventional radical grafting techniques, especially in regard to control of the length of polymer chains attached to the surface of nonporous materials. ATRP also allows for the precision control of three-dimensional architecture required for high resolution separation of intact proteins. I will describe the range of synthetic tools used in the preparation of such materials and demonstrate the advantages that ATRP provides in the preparation of high resolution chromatographic media. 

Application of ATRP to the synthesis of nonporous chromatographic media optimized for the separation of proteins

248th National Meeting (2014)

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