Glycation is a series of non-enzymatic reaction induced by the amine from protein and the carbonyl compound from reducing sugars. The intermediate products, reactive dicarbonyl species (RCS), of the reaction will accelerate the glycation rate. It has been shown that advanced glycation end-products (AGEs) cause aging and other chronic diseases. In this study, we investigated the monomer composition of the proanthocyanidins structure, inhibition activity on the regulation of blood sugar digestion enzyme and the anti-glycation activity in bovine serum albumin (BSA)-fructose system from extract of the immature persimmon fruit (Diospyros kaki L. cv. Bull heart). After using benzyl mercaptan and phloroglucinol as nucleophilic reagents for degradation of the immature persimmon extract (IPE), the result indicated that the terminal unit of proanthocyanidins is (-)-epicatechin (EC). By LC-MS/MS fragment ion chromatograms, three possible extension units were identified, including (-)-epigallocatechin (EGC), (-)-epigallocatechin-3-O-gallate (EGCG) and (-)-epicatechin-3-O-gallate (ECG). The inhibition activity of IPE on the a-glucosidase was significantly higher than the positive control group, acarbose. Based on RCS trapping study, IPE was able to react with methylglyoxal (MGO) in phosphate buffer solution at pH 7.4. Our results indicated that by delaying the carbohydrate hydrolysis and anti-glycation activity, IPE can prevent the complications of diabetes and glycation related chronic diseases.

Anti-glycation activities of immature persimmon fruit (<i>Diospyros kaki</i> L. cv. Bull heart) extract

248th National Meeting (2014)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.

Anti-glycation activities of immature persimmon fruit (Diospyros kaki L. cv. Bull heart) extract