Genetically modified (GM) crops may contain newly expressed proteins with properties that make it extremely difficult or impossible with current methods to express, isolate, purify, or concentrate in heterologous systems are described as �intractable�. Five classes of intractable proteins are discussed here: (1) membrane proteins, (2) signaling proteins, (3) transcription factors, (4) N-glycosylated proteins, and (5) resistance proteins (R-proteins). While the basic tiered weight-of-evidence approach is applicable to intractable proteins, new or modified methods may be required. The extremely low level of expression of most intractable proteins should be taken into account while assessing safety of the intractable protein in GM crops. If Tier II (hazard characterization) analyses requiring animal feeding are judged to be necessary, alternatives to feeding high doses of pure protein may be needed. This presentation will suggest how one can use existing technology and modifications of existing technology to undertake a safety analysis of intractable proteins.

Characteristics and safety assessment of intractable proteins in genetically modified crops

248th National Meeting (2014)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.