Soybean is a multi-billion dollar commodity due to its balanced composition of 2:2:1 protein, starch, and oil by weight. Many seeds, including those of soybeans, contain proteins that are allergens and anti-nutritional factors. As such, there are concerns regarding the potential of altering allergen levels in genetically modified soybean varieties when compared to varieties developed through traditional breeding. A multiplexed LC-MS/MS method was developed and validated for the simultaneous quantitation of allergen proteins in transgenic and non-transgenic soybean. This method has been evaluated for analytical figures of merit including accuracy, precision, linearity, limits of detection, and quantitation; and for other considerations including sample throughput, transferability, and ease of use. Ranges of protein expression levels among various soybean varieties and growing geographies were determined using the described LC-MS/MS technology demonstrating that natural variance and the environment contribute greatest to the variable allergen content in soybean.

Development and implementation of multiplexed LC-MS/MS strategies for the quantitation of endogenous allergens from soybean varieties

248th National Meeting (2014)

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