Since the introduction of genetically modified (GM) crops in the mid-1990s, singleplex ELISA technology has been a primary tool for protein detection in various areas across the agricultural biotechnology industry.  However, it has been challenging to meet high throughput needs for handling large quantity of samples and analyzing fast growing multi-trait stack products. Lately, several next generation multiplexing technologies such as antibody-based Meso Scale Discovery (MSD) technology, bead-based Luminex assay,  and non-antibody based LC/MS method have been actively studied and developed, and are positioned to play an important role for future GM detection. Thoroughly and sufficiently validating these methods is challenging due to its nature, but is critical for their application in agricultural biotechnology. The advancement of these leading multiplexing technologies in GM protein detection in plant tissues will be presented. The method validation requirement and obstacles of its applications in GM products will be discussed in detail.

Multiplexing protein detection technologies for GM crops

248th National Meeting (2014)

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